ABSTRACT The genome-probing microarray (GPM) was developed for quantitative, high-throughput monitoring of community dynamics in lactic acid bacteria (LAB) fermentation through the deposit of 149 microbial genomes as probes on a glass slide. Compared to oligonucleotide microarrays, the specificity of GPM was remarkably increased to a species-specific level. GPM possesses about 10- to 100-fold higher sensitivity (2.5 ng of genomic DNA) than the currently used 50-mer oligonucleotide microarrays. East West Quantum Leap Silk Crack - Torrent. Since signal variation between the different genomes was very low compared to that of cDNA or oligonucleotide-based microarrays, the capacity of global quantification of microbial genomes could also be observed in GPM hybridization. In order to assess the applicability of GPMs, LAB community dynamics were monitored during the fermentation of kimchi, a traditional Korean food.

In this work, approximately 100 diverse LAB species could be quantitatively analyzed as actively involved in kimchi fermentation. Since human beings began eating fermented foods, via “the oldest biotechnology,” lactic acid bacteria (LAB) have been of major concern to microbiologists (,, ). LAB play crucial roles in the manufacturing of fermented milk products, vegetables, and meat as well as in the processing of other products such as wine.